Evaluation of thermo-stability of bluetongue virus recombinant VP7 antigen in indirect ELISA
نویسندگان
چکیده
منابع مشابه
Serological differentiation of five bluetongue virus serotypes in indirect ELISA.
The serological reactivity in indirect ELISA of five different bluetongue virus (BTV) serotypes (4, 10, 15, 16 & 20) was compared using polyclonal antisera raised against virus particles and an outer structural protein, VP2. Rabbit and sheep antisera against BTV-10 produced higher ELISA values with their homologous antigens than with heterologous serotypes. A hyperimmune rabbit serum specific f...
متن کاملComparative Evaluation of c-ELISA and i-ELISA for Detection of Bluetongue Virus Antibodies in Sheep
Bluetongue (BT) is an infectious, noncontagious, arthropod-borne viral disease, principally of sheep but many domestic and wild animals are also affected by this disease (Akita et al., 1994). The severity of BT in sheep varies depending on the virus, the breed of sheep and environmental stress. Infection in cattle and goats is usually sub-clinical. Etiological agent of the disease belongs to th...
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15 صفحه اولEvaluation of an indirect ELISA for detection of antibodies in bulk milk against bluetongue virus infections in the Netherlands.
After the introduction of bluetongue virus serotype 8 (BTV-8) in western Europe in 2006, an indirect ELISA for detection of serogroup-specific antibodies against BTV in serum samples was validated for individual milk samples by the Central Veterinary Institute and the Animal Health Service in the Netherlands (Kramps et al., 2008). In order to develop a cost-effective monitoring tool, we now hav...
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ژورنال
عنوان ژورنال: VirusDisease
سال: 2015
ISSN: 2347-3584,2347-3517
DOI: 10.1007/s13337-014-0244-6